2,740 research outputs found

    Freeze/Thaw-Induced Embolism: Probability of Critical Bubble Formation Depends on Speed of Ice Formation

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    Bubble formation in the conduits of woody plants sets a challenge for uninterrupted water transportation from the soil up to the canopy. Freezing and thawing of stems has been shown to increase the number of air-filled (embolized) conduits, especially in trees with large conduit diameters. Despite numerous experimental studies, the mechanisms leading to bubble formation during freezing have not been addressed theoretically. We used classical nucleation theory and fluid mechanics to show which mechanisms are most likely to be responsible for bubble formation during freezing and what parameters determine the likelihood of the process. Our results confirm the common assumption that bubble formation during freezing is most likely due to gas segregation by ice. If xylem conduit walls are not permeable to the salts expelled by ice during the freezing process, osmotic pressures high enough for air seeding could be created. The build-up rate of segregated solutes in front of the ice-water interface depends equally on conduit diameter and freezing velocity. Therefore, bubble formation probability depends on these variables. The dependence of bubble formation probability on freezing velocity means that the experimental results obtained for cavitation threshold conduit diameters during freeze/thaw cycles depend on the experimental setup; namely sample size and cooling rate. The velocity dependence also suggests that to avoid bubble formation during freezing trees should have narrow conduits where freezing is likely to be fast (e.g., branches or outermost layer of the xylem). Avoidance of bubble formation during freezing could thus be one piece of the explanation why xylem conduit size of temperate and boreal zone trees varies quite systematically.We are grateful for support from the Andrew W. Mellon Foundation and the Materials Research Science and Engineering Center at Harvard University. We also thank the Australian Research Council for support (DP110105380)

    Book Reviews

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    Equity, an Analysis of Modern Equity Problems Designed Primarily for Students. George L. Clark, SJ.D., Professor of Law, University of Missouri. E. W. Stephens Publishing Co., Columbia, Mo., igig. Pp. lii, 639

    Ion Induced Changes in the Structure of Bordered Pit Membranes

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    Ion-mediated changes in xylem hydraulic resistance are hypothesized to result from hydrogel like properties of pectins located in the bordered pit membranes separating adjacent xylem vessels. Although the kinetics of the ion-mediated changes in hydraulic resistance are consistent with the swelling/deswelling behavior of pectins, there is no direct evidence of this activity. In this report we use atomic force microscopy (AFM) to investigate structural changes in bordered pit membranes associated with changes in the ionic concentration of the surrounding solution. When submerged in de-ionized water, AFM revealed bordered pit membranes as relatively smooth, soft, and lacking any sharp edges surface, in contrast to pictures from scanning electron microscope (SEM) or AFM performed on air-dry material. Exposure of the bordered pit membranes to 50 mM KCl solution resulted in significant changes in both surface physical properties and elevation features. Specifically, bordered pit membranes became harder and the fiber edges were clearly visible. In addition, the membrane contracted and appeared much rougher due to exposed microfibers. In neither solution was there any evidence of discrete pores through the membrane whose dimensions were altered in response to the ionic composition of the surrounding solution. Instead the variable hydraulic resistance appears to involve changes in the both the permeability and the thickness of the pit membrane

    The Biotechnology Cluster in Vancouver

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    There is a strong biotechnology cluster in Vancouver yet there is no major manufacturing facility. The Vancouver cluster is unlike other biotech clusters in that its output is solely intellectual property. This ongoing study not only examines the usual structure of a cluster, but also will focus on the issue of intellectual environment, and seek to determine whether a strong cluster can be built without the presence of a large, globally-competitive, manufacturer

    Note and Comment

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    Inducing Breach of Agreement by Employees Not to Join a Labor Union, in Order to Compel Unionization of Plaintiff\u27s Business - In Hitchnan Coal & Coke Compazy v. John Mitchell, et al., (Dec. 10, 1917), 38 Sup. Ct. 6s, the novel question was presented to the Supreme Court of the United States, as to whether or not members of a labor Union could be enjoined from conspiring to persuade, and persuading, without violence or show of violence, plaintiff\u27s employees, not members of the Union,-and who were working for plaintiff not for a specified time, but under an agreement not to continue in plaintiff\u27s employment if they joined the Union, this agreement being fully known to defendants,-secretly to agree to join the Union and continue working for plaintiff until enough had agreed to join, so that a strike could be called, and plaintiff be thereby forced to unionize its business of mining coal

    The redox potential of human Protein Disulphide Isomerase' a' domain, and further characterisation of hPDI's ligand binding behaviour

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    Protein Disulphide Isomerase (PDI) is a 57 kDa multi-domain protein found within the endoplasmic reticulum. PDI consists of four thioredoxin-like domains (named a and b) each containing five ?-sheets and four ?-helices in the conformation ?????????. The four domains are ordered in the sequence abb'xa'c; with a 19 amino acid x linker between the b' and a' domains, and an acidic tail, c, after the a' domain. PDI acts as an oxidoreductase and chaperone to help fold newly synthesized proteins into their native state through the formation of disulphide bonds via the oxidation of sulfhydryl groups of cysteines, as well as the rearrangement of mispaired disulphides in an isomerisation reaction. The catalytic domains responsible for the thiol/disulphide reactions are a and a', and one of the aims of this work is to determine the redox potential of the a' domain and how it is influenced by the adjacent b', x and c regions. Fragments of human PDI containing the a' domain were expressed in E.coli and their redox potential measured by 15N/1H NMR using mixtures of reduced and oxidised glutathione. The b' domain of PDI which is known to contain the primary binding site for unfolded substrates was also investigated. The interaction of the b'xa'c fragment to the PDI ligand ?-somatostatin fused to the carrier protein GB1 was used to investigate its binding behaviour by NMR in a range of different redox conditions

    Assessing student engagement in a multi-media teaching tool in Pharmacy

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    The School of Pharmacy at the University of Waterloo offers an Integrated Patient Focused Care (IPFC) course series to students with the first of nine courses being taught in their second year. IPFC1 includes sections on Pharmacokinetics, Clinical Biochemistry, Immunology, Critical Appraisal and Patient Focused Care. The Pharmacokinetics section is taught using an online and in-class blended approach. A survey of the 2009 cohort demonstrated that this format aided in student learning and that enthusiasm for this section of the course increased over time. A more traditional, lecture-based section of this course, Clinical Biochemistry, was not well received by students; their feedback suggested that they were not able to make clear links between laboratory data and patient assessment. We used student feedback from the 2010 course offering to drive the development of a multi-media online learning module and face-to-face instructor-led tutorial to teach the Clinical Biochemistry section of the course this winter. The design and implementation of the new modules provided us with an opportunity to investigate whether the introduction of multi-media based teaching using virtual field trips, self-assessments and a single face-to-face tutorial increased student understanding of the connections between the results from lab measurements and patient assessment and whether this teaching format enhanced student engagement. We will present how assessment of student learning and engagement was accomplished before and after the introduction of the online components, the results of our analysis of exam grades from both years and our qualitative analysis of students’ feedback from the 2011 class
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